Human Thrombomodulin ELISA Set (without plates) (ab47358)
Overview
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Product name
Human Thrombomodulin ELISA Set (without plates) -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
0.625 ng/ml - 20 ng/mlAssay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s Thrombomodulin Human in vitro ELISA Set is designed for the quantitative measurement of Thrombomodulin in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.
This ELISA Set is intended to measure human Thrombomodulin in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.
These reagents are intended for research use only, not for diagnostic purposes.
The Thrombomodulin ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.
Recognizes both natural and recombinant human Thrombomodulin.
Tested applications
Suitable for: Sandwich ELISAmore detailsPlatform
ReagentsProperties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 5 x 96 tests 10 x 96 tests 15 x 96 tests Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Detection biotinylated anti-Thrombomodulin antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml Streptavidin-HRP 1 vial 2 vials 3 vials Thrombomodulin Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml Thrombomodulin standard 5 vials 10 vials 15 vials -
Research areas
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Relevance
Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. Endothelial cells are unique in synthesizing thrombomodulin. The thrombomodulin/thrombin complex is responsible for the conversion of protein C to the activated protein C (protein Ca), which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Defects in Thrombomodulin may be a cause of thromboembolic disease. -
Cellular localization
single-pass type I membrane protein -
Alternative names
- CD141
- CD141 antigen
- Fetomodulin
see all -
Database links
- Entrez Gene: 7056 Human
- SwissProt: P07204 Human
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 5 x 96 tests 10 x 96 tests 15 x 96 tests Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials Detection biotinylated anti-Thrombomodulin antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml Streptavidin-HRP 1 vial 2 vials 3 vials Thrombomodulin Capture Antibody 1 x 0.5ml 2 x 0.5ml 3 x 0.5ml Thrombomodulin standard 5 vials 10 vials 15 vials -
Research areas
-
Relevance
Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. Endothelial cells are unique in synthesizing thrombomodulin. The thrombomodulin/thrombin complex is responsible for the conversion of protein C to the activated protein C (protein Ca), which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Defects in Thrombomodulin may be a cause of thromboembolic disease. -
Cellular localization
single-pass type I membrane protein -
Alternative names
- CD141
- CD141 antigen
- Fetomodulin
see all -
Database links
- Entrez Gene: 7056 Human
- SwissProt: P07204 Human
Images
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Thrombomodulin Human ELISA Set (ab47358)Thrombomodulin Standard curve ranging from 0.625 to 20 ng/ml