Human Thioredoxin 1 ELISA Kit (ab277448)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.205 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Thioredoxin 1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.2 ng/ml -
Range
0.205 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 98.98 95% - 106% Plasma 93.86 87% - 104% Cell culture media 108.2 100% - 115% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Thioredoxin 1 ELISA Kit (ab277448) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Thioredoxin 1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Thioredoxin 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Thioredoxin 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Thioredoxin 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Thioredoxin 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 1200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human Thioredoxin 1 Antibody 2 vials Anti-Human Thioredoxin 1 coated Microplate 1 unit Human Thioredoxin 1 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/ dithiol group within the conserved Cys-Gly-Pro-Cys active site. Specific protein disulfide targets for reduction by Trx include protein disulfide –isomerase (PDI) and a number of transcription factors such as p53, NFkB and AP-1. Trx is also apable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins. -
Cellular localization
Cytoplasmic -
Alternative names
- ADF
- ATL-derived factor
- SASP
see all -
Database links
- Entrez Gene: 7295 Human
- Omim: 187700 Human
- SwissProt: P10599 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.