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Human STAT2 knockout A549 cell pellet (ab278853)

Overview

  • Product name

    Human STAT2 knockout A549 cell pellet
    See all STAT2 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    A549

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon2 and 1 bp deletion in exon2.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

  • Sequence similarities

    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.

  • Post-translational
    modifications

    Tyrosine phosphorylated in response to IFN-alpha.

  • Cellular localization

    Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
  • Information by UniProt

  • Alternative names

    • Homo sapiens interferon alpha induced transcriptional activator
    • interferon alpha induced transcriptional activator
    • ISGF 3
    • ISGF3
    • MGC59816
    • P113
    • Signal transducer and activator of transcription 2
    • signal transducer and activator of transcription 2 113kD
    • STAT113
    • Stat2
    • STAT2_HUMAN
    see all

Properties

Images

  • Western blot - Human STAT2 knockout A549 cell pellet (ab278853)
    Western blot - Human STAT2 knockout A549 cell pellet (ab278853)

    Lane 1: Wild-type A549 cell lysate (20 µg)

    Lane 2: STAT2 knockout A549 cell lysate (20 µg)

    Lanes 1-2: Merged signal (red and green). Green - ab32367 observed at 97 kDa. Red - loading control ab8245 observed at 37 kDa.

    ab32367 Anti-STAT2 antibody [Y141] was shown to specifically react with STAT2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267006 (knockout cell lysate ab257185) was used. Wild-type and STAT2 knockout samples were subjected to SDS-PAGE. ab32367 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human STAT2 knockout A549 cell pellet (ab278853)
    Sanger Sequencing - Human STAT2 knockout A549 cell pellet (ab278853)

    Allele-1: 19 bp deletion in exon2

     

  • Sanger Sequencing - Human STAT2 knockout A549 cell pellet (ab278853)
    Sanger Sequencing - Human STAT2 knockout A549 cell pellet (ab278853)

    Allele-2: 1 bp deletion in exon2

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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