Human STAT2 knockout A549 cell lysate (ab257183)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab263523 - Human STAT2 knockout A549 cell lysate 1 x 100µg ab255554 - Human wild-type A549 cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Carcinoma -
STR Analysis
Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12
Images
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Western blot - Human STAT2 knockout A549 cell lysate (ab257183)
Lane 1: Wild-type A549 cell lysate 20 ug
Lane 2: STAT2 knockout A549 cell lysate 20 ug
Lanes 1 - 2:Merged signal (red and green). Green - ab32367 observed at 97 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab32367 was shown to react with STAT2 in wild-type A549 cells in western blot with loss of signal observed in STAT2 knockout cell line ab267004 (STAT2 knockout cell lysate ab257183). Wild-type and STAT2 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk before incubation with ab32367 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging. -
Sanger Sequencing - Human STAT2 knockout A549 cell lysate (ab257183)Allele-1: 2 bp deletion in exon2
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Sanger Sequencing - Human STAT2 knockout A549 cell lysate (ab257183)Allele-2: 1 bp deletion in exon2