Human SNCA (Alpha-synuclein) knockout HEK-293T cell lysate (ab263769)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab255537 - Human SNCA knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human SNCA knockout HEK293T cell lysate (ab263769)
Lane 1: Wild-type HEK-293T cell lysate (20ug)
Lane 2: SNCA knockout HEK-293T cell lysate (20ug)Lanes 1- 2: Merged signal (red and green). Green - ab138501 observed at 18 kDa. Red - loading control ab8245 observed at 37 kDa.
ab138501 Recombinant Anti-Alpha-synuclein antibody [MJFR1] was shown to specifically react with SNCA in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab255433 (knockout cell lysate ab263769) was used. Wild-type and SNCA knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab138501 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human SNCA knockout HEK293T cell lysate (ab263769)Allele-1: Insertion of the selection cassette in exon2; Allele-2: 1 bp insertion in exon2
