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Human SMAD5 Matched Antibody Pair Kit (ab218179)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Human
  • Range: 62.5 pg/ml - 4000 pg/ml

Overview

  • Product name

    Human SMAD5 Matched Antibody Pair Kit
    See all SMAD5 kits

  • Detection method

    Colorimetric

  • Assay type

    ELISA set

  • Sensitivity

    27.78 pg/ml

  • Range

    62.5 pg/ml - 4000 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human SMAD5 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human SMAD5.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab213471. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Human SMAD5 Capture Antibody 2 x 50µg 1 x 50µg
    Human SMAD5 Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Human SMAD5 Lyophilized Protein 2 vials 1 vial

  • Research areas

  • Function

    Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.

  • Post-translational
    modifications

    Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
    Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.

  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
  • Information by UniProt

  • Alternative names

    • DKFZp781C1895
    • DKFZp781O1323
    • Dwfc
    • hSmad5
    • JV5 1
    • JV5-1
    • MAD homolog 5
    • MAD, mothers against decapentaplegic homolog 5
    • MADH 5
    • MADH5
    • Mothers against decapentaplegic homolog 5
    • mothers against decapentaplegic, drosophila, homolog of, 5
    • Mothers against DPP homolog 5
    • MusMLP
    • SMA and MAD related protein 5
    • SMAD 5
    • SMAD family member 5
    • SMAD, mothers against DPP homolog 5
    • Smad5
    • SMAD5_HUMAN
    see all

  • Database links

    Images

    • Human SMAD5 standard curve.
      Human SMAD5 standard curve.

      Standard calibration curve. Background subtracted values are graphed.

    • Sandwich ELISA - Human SMAD5 Matched Antibody Pair Kit (ab218179)
      Sandwich ELISA - Human SMAD5 Matched Antibody Pair Kit (ab218179)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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