Human Serpin A4 ELISA Kit (ab193714)
Key features and details
- Sensitivity: 0.12 ng/ml
- Range: 0.12 ng/ml - 35 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Serpin A4 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.12 ng/ml -
Range
0.12 ng/ml - 35 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 108.3 87% - 115% Plasma 110.4 102% - 120% Cell culture media 106.3 100% - 113% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Serpin A4 Human ELISA Kit (ab193714) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Serpin A4 in serum, plasma, and cell culture supernatant.
This assay employs an antibody specific for human Serpin A4 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Serpin A4 present in the samples. The wells are washed and biotinylated anti-Human Serpin A4 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Serpin A4 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 600X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated Human Serpin A4 detection antibody (lyophilized) 2 vials Human Serpin A4 Standards (lyophilized) 2 vials Pre-coated Human Serpin A4 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- KAIN_HUMAN
- KAL
- Kallikrein binding protein
see all -
Database links
- Entrez Gene: 5267 Human
- Omim: 147935 Human
- SwissProt: P29622 Human
- Unigene: 719893 Human
Images
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Typical Standard Curve
Example of typical Human Serpin A4 standard curve in Assay Diluent A and B.
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Standard curve