Human ROR1 ELISA Kit (ab267606)
Key features and details
- Sensitivity: 0.082 ng/ml
- Range: 0.819 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ROR1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.082 ng/ml -
Range
0.819 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 73.36 70% - 78% Plasma 71.44 68% - 79% Cell culture media 72.05 69% - 81% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human ROR1 ELISA Kit is designed for the quantitative determination of ROR1 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human ROR1 coated on a 96-well plate. Standards and samples are pipetted into the wells and ROR1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ROR1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ROR1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human ROR1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human ROR1 Detection Antibody 2 vials Human ROR1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Tyrosine-protein kinase receptor whose role is not yet clear. -
Tissue specificity
Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.
Contains 1 FZ (frizzled) domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 kringle domain.
Contains 1 protein kinase domain. -
Developmental stage
Expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- dJ537F10.1
- Inactive tyrosine protein kinase transmembrane receptor ROR1
- MGC99659
see all -
Database links
- Entrez Gene: 4919 Human
- Omim: 602336 Human
- SwissProt: Q01973 Human
- Unigene: 654491 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.