Human ROCK1 knockout HeLa cell lysate (ab257642)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260312 - Human ROCK1 knockout HeLa cell lysate 1 x 100µg ab255552 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human ROCK1 knockout HeLa cell lysate (ab257642)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: ROCK1 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab134181 observed at 160 kDa. Red - loading control, ab7291 observed at 50 kDa.
ab134181 Anti-ROCK1 antibody [EPR638Y] was shown to specifically react with ROCK1 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab264780 (knockout cell lysate ab257642) lane below 160kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and ROCK1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab134181 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human ROCK1 knockout HeLa cell lysate (ab257642)Homozygous: Insertion of the selection cassette in exon 4