Microplate wells are pre-coated with anti-human RNAse 7-specific monoclonal capture antibodies. Samples and standards are pipetted into microwells and human RNAse 7 molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated human RNAse 7-specific monoclonal detection antibodies bind to a different epitope of human RNase 7 molecules. After washing, the ready-to-use HRP substrate (TMB) is added to the wells. The intensity of the color produced is directly proportional to the amount of human RNAse 7 in the sample. Color development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm.