Human Ribonuclease 3 ELISA Kit (ECP) (ab279415)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.265 pg/ml
- Range: 9.375 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Ribonuclease 3 ELISA Kit (ECP) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.6% Inter-assay Sample n Mean SD CV% Serum 3 6.8% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.265 pg/ml -
Range
9.375 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 98 93% - 103% Serum 101 94% - 109% Hep Plasma 93 91% - 94% EDTA Plasma 100 97% - 104% Cit plasma 88 86% - 91% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Ribonuclease 3 ELISA kit (ab279415) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Ribonuclease 3 protein in human serum, plasma, cellculture supernatant and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Ribonuclease 3 with 1.27 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Ribonuclease 3, also known as Eosinophil Cationic Protein (ECP) is part of the Ribonuclease A family and is able to degrade RNA. Ribonuclease 3 is expressed by the eosinophils and secreted into the blood stream. Serum and plasma levels of Ribonuclease 3 increase in response to inflammatory diseases. Ribonuclease 3 is measured diagnostically to diagnose allergic disorders and other conditions connected with activated eosinophils.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Ribonuclease 3 (ECP) Capture Antibody 1 x 600µl 10X Human Ribonuclease 3 (ECP) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human Ribonuclease 3 (ECP) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Function
Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity. -
Sequence similarities
Belongs to the pancreatic ribonuclease family. -
Cellular localization
Cytoplasmic granule. Matrix of eosinophil's large specific granule. - Information by UniProt
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Alternative names
- Cytotoxic ribonuclease
- ECP
- ECP_HUMAN
see all -
Database links
- Entrez Gene: 6037 Human
- Omim: 131398 Human
- SwissProt: P12724 Human
- Unigene: 73839 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody benefitsTo learn more about the advantages of recombinant antibodies see here.