Human PXN (Paxillin) knockout HeLa cell line (ab264767)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Cervix -
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10 -
Antibiotic resistance
Puromycin 1.00µg/ml -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Research areas
Images
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Western blot - Human PXN knockout HeLa cell line (ab264767)All lanes : Anti-Paxillin antibody [E228] (ab32115) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PXN knockout HeLa cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDaLanes 1-4: Merged signal (red and green). Green - ab32115 observed at 65 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32115 Anti-Paxillin antibody [E228] was shown to specifically react with Paxillin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264767 (knockout cell lysate ab257044) was used. Wild-type and Paxillin knockout samples were subjected to SDS-PAGE. ab32115 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Human PXN knockout HeLa cell line (ab264767)All lanes : Anti-Paxillin antibody [Y113] (ab32084) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PXN knockout HeLa cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab32084 observed at 68 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32084 Anti-Paxillin antibody [Y113] was shown to specifically react with Paxillin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264767 (knockout cell lysate ab257044) was used. Wild-type and Paxillin knockout samples were subjected to SDS-PAGE. ab32084 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human PXN knockout HeLa cell line (ab264767)Homozygous: 1 bp insertion in exon 4.
