Human PTPN11 (SHP2) knockout HEK293T cell pellet (ab278893)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human PTPN11 knockout HEK293T cell pellet 1 vial Human wild-type HEK293T cell pellet 1 vial -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human PTPN11 (SHP2) knockout HEK293T cell pellet (ab278893)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: PTPN11 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab32083 observed at 68 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32083 Anti-SHP2 antibody [Y478] was shown to specifically react with SHP2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266450 (knockout cell lysate ab257618) was used. Wild-type and SHP2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32083 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human PTPN11 (SHP2) knockout HEK293T cell pellet (ab278893)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: PTPN11 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab187040 observed at 68 kDa. Red - loading control ab8245 observed at 37 kDa.
ab187040 Anti-SHP2 antibody [EPR17829-9] was shown to specifically react with SHP2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266450 (knockout cell lysate ab257618) was used. Wild-type and SHP2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab187040 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human PTPN11 (SHP2) knockout HEK293T cell pellet (ab278893)Allele-1: 2 bp deletion in exon1
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Sanger Sequencing - Human PTPN11 (SHP2) knockout HEK293T cell pellet (ab278893)Allele-2: 1 bp insertion in exon1