Human PSA ELISA Kit (ab264615)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8 pg/ml
- Range: 39 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PSA ELISA Kit
See all Prostate Specific Antigen kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Urine 8 5.9% Inter-assay Sample n Mean SD CV% Urine 3 8.4% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8 pg/ml -
Range
39 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 92 86% - 97% Urine 116 112% - 120% Serum 99 95% - 103% Hep Plasma 86 85% - 88% EDTA Plasma 88 80% - 94% Cit plasma 115 108% - 120% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human PSA ELISA Kit (ab264615) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PSA protein in edta plasma, hep plasma, serum, fetal bovine serum, urine, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PSA with 8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Prostate specific antigen (PSA) or also commonly termed tissue Kallikrein-3 (KLK3) is a 34 kD serine protease from the Kallikrein family. PSA has very high expression in the prostate gland, but expression also has been reported in the breast and saliva. PSA functions by hydrolyzing semenogelin-1 leading to the liquefaction of the seminal coagulum. This is essential for the proper release of sperm during ejaculation. The majority (>70%) of PSA is known to form a complex with alpha-1-antichymotrypsin (PSA-ACT) in the blood. The remaining PSA exists as an unbound inactive enzyme known as Free PSA. PSA is best characterized as a clinical biomarker for Prostate Cancer. During prostate cancer progression, excess amounts of PSA are leaked into the blood dramatically elevating both Total and Free PSA levels. This assay detects total PSA levels.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human PSA Capture Antibody 1 x 600µl 10X Human PSA Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human PSA Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- antigen, prostate-specific
- APS
- Gamma seminoprotein
see all -
Database links
- Entrez Gene: 354 Human
- Omim: 176820 Human
- SwissProt: P07288 Human
- Unigene: 171995 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human PSA standard curve comparisonStandard Curve comparison between human PSA SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human PSA standard curve in Sample Diluent NS.The PSA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PSA in human serum and plasma (citrate) samples.The concentrations of PSA were measured in duplicates, interpolated from the PSA standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSA concentration was determined to be 630 pg/mL in serum, 1,300 pg/mL in plasma (citrate).
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Interpolated concentrations of native PSA in human male urine samples.The concentrations of PSA were measured in duplicates, interpolated from the PSA standard curves and corrected for sample dilution. Undiluted samples are as follows: male urine 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSA concentration was determined to be 174 ng/mL in male urine.
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Serum from eight individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSA concentration was determined to be 826 pg/mL with a range of 234 – 2,201 pg/mL.
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Urine from both pooled male and female donors was measured in duplicate at 1:200 dilution.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Female urine was below the lower limit of detection and 174 ng/mL in male urine.
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Sandwich ELISA - Human PSA ELISA Kit (ab264615)To learn more about the advantages of recombinant antibodies see here.
