Human PR3 ELISA Kit (ab226902)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 150 pg/ml
- Range: 1.2 ng/ml - 150 ng/ml
- Sample type: Cell culture extracts, Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PR3 ELISA Kit
See all PR3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 3.7% Inter-assay Sample n Mean SD CV% Supernatant 3 11% -
Sample type
Cell culture supernatant, Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
150 pg/ml -
Range
1.2 ng/ml - 150 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 101 90% - 117% Cell culture extracts 89 87% - 93% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human PR3 ELISA Kit (ab226902) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PR3 protein in cell culture extracts and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PR3 with 150 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml 10X Human PR3 Capture Antibody 1 x 600µl 10X Human PR3 Detector Antibody 1 x 600µl Human PR3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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Alternative names
- ACPA
- AGP 7
- AGP7
see all -
Database links
- Entrez Gene: 5657 Human
- Omim: 177020 Human
- SwissProt: P24158 Human
- Unigene: 928 Human
Images
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Other - Human PR3 ELISA Kit (ab226902)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of HumanPR3 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of HumanPR3 standard curve in Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PR3 in Human cell extracts and cell culture supernatant samplesThe concentrations of PR3 were measured in duplicates, interpolated from the PR3 standard curves and corrected for sample dilution. Undiluted samples are as follows: THP-1 cell extract (100 g/ml); HL-60 (50 g/ml); untreated THP-1 cell supernatant (100 %) and untreated Human PBMC supernatant (50%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PR3 concentration was determined to be 24.62 ng/mL in THP-1 cell extract, 72.65 ng/mL in HL-60 cell extract and 6.16 ng/mL in THP-1 supernatant and 146.21 ng/ml in PBMC supernatant.
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Sandwich ELISA - Human PR3 ELISA Kit (ab226902)To learn more about the advantages of recombinant antibodies see here.