Human PPIF (Cyclophilin F) knockout HEK-293T cell lysate (ab257039)
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260168 - Human PPIF knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
-
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
-
Western blot - Human PPIF (Cyclophilin F) knockout HEK293T cell lysate (ab257039)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: PPIF knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab110324 observed at 23 kDa. Red - loading control ab181602 observed at 37 kDa.
ab110324 Anti-Cyclophilin F antibody [E11AE12BD4] was shown to specifically react with Cyclophilin F in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266077 (knockout cell lysate ab257039) was used. Wild-type and Cyclophilin F knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab110324 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human PPIF (Cyclophilin F) knockout HEK293T cell lysate (ab257039)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: PPIF knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab231155 observed at 23 kDa. Red - loading control ab8245 observed at 37 kDa.
ab231155 Anti-Cyclophilin F antibody [EPR11311-121] was shown to specifically react with Cyclophilin F in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266077 (knockout cell lysate ab257039) was used. Wild-type and Cyclophilin F knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab231155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human PPIF knockout HEK293T cell lysate (ab257039)Homozygous: 1 bp insertion in exon 1
