Human PMN Elastase ELISA Kit (ab119553)
Key features and details
- Sensitivity: 1.98 pg/ml
- Range: 0.16 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PMN Elastase ELISA Kit
See all Neutrophil Elastase kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.8% Inter-assay Sample n Mean SD CV% Overall 5.6% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.98 pg/ml -
Range
0.16 ng/ml - 10 ng/ml -
Recovery
104 %
Sample specific recovery Sample type Average % Range Plasma 104 96% - 110% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s PMN Elastase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human PMN Elastase concentrations in Cell culture supernatants, plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma.
PMN Elastase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated PMN Elastase detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the PMN Elastase amount of sample captured in plate.
The capture antibody in the Human PMN-Elastase ELISA kit is specific to PMN elastase, while the detection antibody is specific to alpha1-PI, hence the kit is specific to the PMN Elastase/alpha1-PI complex.
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Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests Adhesive Films 2 units Control high 1 vial Control low 1 vial HRP-Conjugate anti-alpha1-PI polyclonal antibody 1 x 16ml Human PMN Elastase - alpha1-PI complex Standard, lyophilized, (10 ng/mL upon reconstitution) 1 vial Microplate coated with polyclonal antibody to Human PMN Elastase (12 x 8 wells) 1 unit Sample Diluent 1 x 50ml Stop Solution 1 x 7ml TMB Substrate Solution 1 x 22ml Wash Buffer Concentrate (10x) 1 x 50ml -
Research areas
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Function
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. -
Tissue specificity
Bone marrow cells. -
Involvement in disease
Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. -
Sequence similarities
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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Alternative names
- Bone marrow serine protease
- ELA2
- ELANE
see all -
Database links
- Entrez Gene: 1991 Human
- Omim: 130130 Human
- SwissProt: P08246 Human
- Unigene: 99863 Human
Images
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ELISA: PMN Elastase Human ELISA Kit (ab119553)
Standard curve with background signal subtracted (duplicates; +/- SD).
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ELISA: PMN Elastase Human ELISA Kit (ab119553)PMN elastase measured in the high control and low control (ab119553) and in biological fluids (dilution range 1/1-1/1000; duplicates +/- SD).
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Typical Standard CurveRepresentative Standard Curve using ab119553.
