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Signal Transduction Metabolism Energy Metabolism

Human PFKFB2 Matched Antibody Pair Kit (ab218805)

Human PFKFB2 Matched Antibody Pair Kit (ab218805)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
  • For economical ELISA and ELISA-based assay development
  • Reacts with: Human
  • Range: 125 pg/ml - 8000 pg/ml

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Overview

  • Product name

    Human PFKFB2 Matched Antibody Pair Kit
    See all PFKFB2 kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Sensitivity

    3.45 pg/ml
  • Range

    125 pg/ml - 8000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human PFKFB2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human PFKFB2.


    Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab214574. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 5 x 96 tests
    Mouse PFKFB2 Capture Antibody 2 x 50µg 1 x 50µg
    Mouse PFKFB2 Detector Antibody 2 x 12.5µg 1 x 12.5µg
    Mouse PFKFB2 Lyophilized Protein 2 vials 1 vial
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
  • Function

    Synthesis and degradation of fructose 2,6-bisphosphate.
  • Tissue specificity

    Heart.
  • Sequence similarities

    In the C-terminal section; belongs to the phosphoglycerate mutase family.
  • Target information above from: UniProt accession O60825 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 6 phosphofructo 2 kinase/fructose 2 6 biphosphatase 2
    • 6-bisphosphatase
    • 6-P2ase 2
    • 6-P2ASE heart-type isozyme
    • 6PF 2 K/Fru 2 6 P2ASE heart type isozyme
    • 6PF-2-K/Fru-2
    • 6PF-2-K/Fru-26-P2ase 2
    • 6PF-2-K/Fru-26-P2ase heart-type isozyme
    • F262_HUMAN
    • Fructose 2 6 bisphosphatase cardiac isozyme
    • Fructose-2
    • PFK 2/FBPase 2
    • PFK/FBPase 2
    • PFKFB cardiac
    • PFKFB2
    see all
  • Database links

    • Entrez Gene: 5208 Human
    • Entrez Gene: 5207 Human
    • Entrez Gene: 5210 Human
    • Entrez Gene: 5209 Human
    • Omim: 171835 Human
    • SwissProt: O60825 Human
    • SwissProt: P16118 Human
    • SwissProt: Q16877 Human
    • SwissProt: Q16875 Human
    • Unigene: 282702 Human
    • Unigene: 444304 Human
    • Unigene: 476217 Human
    • Unigene: 195471 Human
    see all

Images

  • Human PFKFB2 standard curve
    Human PFKFB2 standard curve

    Standard calibration curve. Background subtracted values are graphed.

  • Sandwich ELISA - Human PFKFB2 Matched Antibody Pair Kit (ab218805)
    Sandwich ELISA - Human PFKFB2 Matched Antibody Pair Kit (ab218805)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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