Human Peroxiredoxin 2/PRP ELISA Kit (ab277456)
Key features and details
- Sensitivity: 0.34 ng/ml
- Range: 0.328 ng/ml - 80 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Peroxiredoxin 2/PRP ELISA Kit
See all Peroxiredoxin 2/PRP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.34 ng/ml -
Range
0.328 ng/ml - 80 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 101.4 92% - 115% Plasma 89.91 82% - 99% Cell culture media 116.8 106% - 130% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Peroxiredoxin 2/PRP ELISA Kit (ab277456) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Peroxiredoxin 2/PRP in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Peroxiredoxin 2/PRP coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Peroxiredoxin 2/PRP present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Peroxiredoxin 2/PRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Peroxiredoxin 2/PRP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human Peroxiredoxin 2/PRP Antibody 2 vials Anti-Human Peroxiredoxin 2 PRP coated Microplate 1 unit Human Peroxiredoxin 2/PRP standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). -
Sequence similarities
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Epididymis secretory sperm binding protein Li 2a
- HEL S 2a
- MGC4104
see all -
Database links
- Entrez Gene: 7001 Human
- Omim: 600538 Human
- SwissProt: P32119 Human
- Unigene: 432121 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.