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Human PEDF ELISA Kit (SerpinF1) (ab213815)

Key features and details

  • Sensitivity: 20 pg/ml
  • Range: 0.78 ng/ml - 50 ng/ml
  • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human PEDF coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
    Biotinylated anti-Human PEDF antibody 1 x 130µl
    Lyophilized recombinant Human PEDF standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml

  • Research areas

  • Function

    Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

  • Tissue specificity

    Retinal pigment epithelial cells and blood plasma.

  • Sequence similarities

    Belongs to the serpin family.

  • Developmental stage

    Expressed in quiescent cells.

  • Domain

    The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.

  • Post-translational
    modifications

    The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

  • Cellular localization

    Secreted. Melanosome. Enriched in stage I melanosomes.
  • Information by UniProt

  • Alternative names

    • Cell proliferation-inducing gene 35 protein
    • EPC 1
    • EPC-1
    • EPC1
    • OI12
    • OI6
    • PEDF
    • PEDF_HUMAN
    • PIG 35
    • PIG35
    • Pigment epithelium derived factor
    • Pigment epithelium-derived factor
    • Proliferation inducing protein 35
    • Serine (or cysteine) proteinase inhibitor
    • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
    • Serpin F1
    • Serpin family F member 1
    • Serpin peptidase inhibitor
    • Serpin peptidase inhibitor clade F member 1
    • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
    • SERPINF 1
    • Serpinf1
    see all

  • Database links

    Images

    • Human PEDF ELISA Kit (SerpinF1) (ab213815) Standard Curve
      Human PEDF ELISA Kit (SerpinF1) (ab213815) Standard Curve

      Human PEDF ELISA Kit (SerpinF1) (ab213815) Standard Curve

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