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Human PAPP A2 ELISA Kit (ab277446)

Key features and details

  • Sensitivity: 0.23 ng/ml
  • Range: 0.2 ng/ml - 150 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human PAPP A2 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.23 ng/ml

  • Range

    0.2 ng/ml - 150 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 117.6 95% - 140%
    Plasma 132.1 127% - 140%
    Cell culture extracts 103 76% - 142%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human PAPP A2 ELISA Kit (ab277446) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PAPP A2 in serum (human PAPP A2 concentration is low in normal serum/plasma, and may not be detectable in this assay concentration), plasma and cell culture supernatants.


    This assay employs an antibody specific for Human PAPP A2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human PAPP A2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human PAPP A2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human PAPP A2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    800X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated Anti-Human PAPP A2 Antibody 2 vials
    Anti-Human PAPP A2 coated Microplate 1 unit
    Human PAPP A2 standard protein (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Function

    Metalloproteinase which specifically cleaves IGFBP-5. Shows limited proteolysis toward IGFBP-3.

  • Tissue specificity

    Expressed abundantly in placenta, and non-pregnant mammary gland with low expression in the kidney, fetal brain and pancreas.

  • Sequence similarities

    Belongs to the peptidase M43B family.
    Contains 5 Sushi (CCP/SCR) domains.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • PAPPA 2
    • PAPP E
    • PAPP-A2
    • PAPP-E
    • PAPP2_HUMAN
    • PAPPA2
    • Pappalysin 2
    • Pappalysin-2
    • PLAC3
    • Pregnancy associated plasma protein A2
    • Pregnancy associated plasma protein E1
    • Pregnancy-associated plasma protein A2
    • Pregnancy-associated plasma protein E1
    see all

  • Database links

    Images

    • Example data
      Example data

      These standard curves are for demonstration only. A standard curve must be run with each assay.

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