Human PAPP A2 ELISA Kit (ab277446)
Key features and details
- Sensitivity: 0.23 ng/ml
- Range: 0.2 ng/ml - 150 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PAPP A2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.23 ng/ml -
Range
0.2 ng/ml - 150 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 117.6 95% - 140% Plasma 132.1 127% - 140% Cell culture extracts 103 76% - 142% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human PAPP A2 ELISA Kit (ab277446) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PAPP A2 in serum (human PAPP A2 concentration is low in normal serum/plasma, and may not be detectable in this assay concentration), plasma and cell culture supernatants.
This assay employs an antibody specific for Human PAPP A2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human PAPP A2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human PAPP A2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human PAPP A2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated Anti-Human PAPP A2 Antibody 2 vials Anti-Human PAPP A2 coated Microplate 1 unit Human PAPP A2 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Metalloproteinase which specifically cleaves IGFBP-5. Shows limited proteolysis toward IGFBP-3. -
Tissue specificity
Expressed abundantly in placenta, and non-pregnant mammary gland with low expression in the kidney, fetal brain and pancreas. -
Sequence similarities
Belongs to the peptidase M43B family.
Contains 5 Sushi (CCP/SCR) domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- PAPPA 2
- PAPP E
- PAPP-A2
see all -
Database links
- Entrez Gene: 60676 Human
- SwissProt: Q9BXP8 Human
- Unigene: 187284 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.
