Human NPTXR ELISA Kit (ab277467)
Key features and details
- Sensitivity: 0.4 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human NPTXR ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.4 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 73.88 70% - 78% Plasma 75.32 68% - 90% Cell culture media 78.46 68% - 94% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human NPTXR ELISA Kit (ab277467) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NPTXR in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human NPTXR coated on a 96-well plate. Standards and samples are pipetted into the wells and Human NPTXR present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human NPTXR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human NPTXR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated Anti-Human NPTXR Antibody 2 vials Anti-Human NPTXR coated Microplate 1 unit Human NPTXR standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses. -
Sequence similarities
Contains 1 pentaxin domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Neuronal pentraxin receptor
- NPR
- Nptxr
- NPTXR_HUMAN
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Database links
- Entrez Gene: 23467 Human
- Omim: 609474 Human
- SwissProt: O95502 Human
- Unigene: 91622 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.