Human Nodal ELISA Kit (ab256391)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.32 ng/ml
- Range: 0.781 ng/ml - 50 ng/ml
- Sample type: Cell culture media, Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Nodal ELISA Kit
See all Nodal kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 8.4% Inter-assay Sample n Mean SD CV% Supernatant 3 8.9% -
Sample type
Cell culture supernatant, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
0.32 ng/ml -
Range
0.781 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 88 86% - 91% Cell culture media 97 95% - 99% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Nodal ELISA Kit (ab256391) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Nodal protein in cell culture media and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Nodal with 0.32 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human Nodal homolog is a secreted member of the TGF-beta superfamily of proteins encoded by the gene NODAL. Nodal binds to Type I and II serine/threonine kinase receptors, which recruits members of the SMAD family of transcription factors. Nodal signaling regulates mesoderm formation and axial patterning during embryonic development.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Nodal Capture Antibody 1 x 600µl 10X Human Nodal Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Nodal Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Essential for mesoderm formation and axial patterning during embryonic development. -
Involvement in disease
Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- HTX5
- MGC138230
- Nodal
see all -
Database links
- Entrez Gene: 4838 Human
- Omim: 601265 Human
- SwissProt: Q96S42 Human
- Unigene: 370414 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Nodal standard curve in Sample Diluent NS.The Nodal standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Nodal in mouse cell culture supernatant samples.The concentrations of Nodal were measured in duplicates, interpolated from the Nodal standard curves and corrected for sample dilution. Undiluted samples are as follows: F9 cell supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nodal concentration was determined to be 9.7 ng/mL in F9 supernatant.
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Sandwich ELISA - Human Nodal ELISA Kit (ab256391)To learn more about the advantages of recombinant antibodies see here.
