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Human Nodal ELISA Kit (ab256391)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 0.32 ng/ml
  • Range: 0.781 ng/ml - 50 ng/ml
  • Sample type: Cell culture media, Cell culture supernatant
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Nodal ELISA Kit
    See all Nodal kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Supernatant 8 8.4%
    Inter-assay
    Sample n Mean SD CV%
    Supernatant 3 8.9%

  • Sample type

    Cell culture supernatant, Cell culture media

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.32 ng/ml

  • Range

    0.781 ng/ml - 50 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 88 86% - 91%
    Cell culture media 97 95% - 99%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Nodal ELISA Kit (ab256391) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Nodal protein in cell culture media and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Nodal with 0.32 ng/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Human Nodal homolog is a secreted member of the TGF-beta superfamily of proteins encoded by the gene NODAL. Nodal binds to Type I and II serine/threonine kinase receptors, which recruits members of the SMAD family of transcription factors. Nodal signaling regulates mesoderm formation and axial patterning during embryonic development.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human Nodal Capture Antibody 1 x 600µl
    10X Human Nodal Detector Antibody 2 vials
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 5BI 1 x 6ml
    Human Nodal Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Essential for mesoderm formation and axial patterning during embryonic development.

  • Involvement in disease

    Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.

  • Sequence similarities

    Belongs to the TGF-beta family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • HTX5
    • MGC138230
    • Nodal
    • Nodal Growth Differentiation Factor
    • Nodal homolog
    • nodal homolog (mouse)
    • Nodal, Mouse, Homolog
    • NODAL_HUMAN
    see all

  • Database links

    Images

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • Example of human Nodal standard curve in Sample Diluent NS.
      Example of human Nodal standard curve in Sample Diluent NS.

      The Nodal standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native Nodal in mouse cell culture supernatant samples.
      Interpolated concentrations of native Nodal in mouse cell culture supernatant samples.

      The concentrations of Nodal were measured in duplicates, interpolated from the Nodal standard curves and corrected for sample dilution. Undiluted samples are as follows: F9 cell supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Nodal concentration was determined to be 9.7 ng/mL in F9 supernatant.

    • Sandwich ELISA - Human Nodal ELISA Kit (ab256391)
      Sandwich ELISA - Human Nodal ELISA Kit (ab256391)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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