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Human MPDU1 (SL15) knockout HEK-293T cell lysate (ab258968)

Overview

  • Product name

    Human MPDU1 (SL15) knockout HEK-293T cell lysate

  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Required for normal utilization of mannose-dolichol phosphate (Dol-P-Man) in the synthesis of N-linked and O-linked oligosaccharides and GPI anchors.

  • Involvement in disease

    Defects in MPDU1 are the cause of congenital disorder of glycosylation type 1F (CDG1F) [MIM:609180]. CDGs are a family of severe inherited diseases caused by a defect in protein N-glycosylation. They are characterized by under-glycosylated serum proteins. These multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.

  • Sequence similarities

    Belongs to the MPDU1 (TC 2.A.43.3) family.
    Contains 2 PQ-loop domains.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • CDGIF
    • LEC35
    • mannose P dolichol utilization defect 1
    • Mannose-P-dolichol utilization defect 1 protein
    • MPDU1
    • MPU1_HUMAN
    • PQLC5
    • SL15
    • Suppressor of Lec15 and Lec35 glycosylation mutation homolog
    see all

Properties

Images

  • Sanger Sequencing - Human MPDU1 knockout HEK293T cell lysate (ab258968)
    Sanger Sequencing - Human MPDU1 knockout HEK293T cell lysate (ab258968)

    Allele-1: 2 bp deletion in exon 1

     

  • Sanger Sequencing - Human MPDU1 knockout HEK293T cell lysate (ab258968)
    Sanger Sequencing - Human MPDU1 knockout HEK293T cell lysate (ab258968)

    Allele-2: Insertion of the selection cassette in exon 1

     

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