Human MEP1A ELISA Kit (ab267627)
Key features and details
- Sensitivity: 2 ng/ml
- Range: 1.638 ng/ml - 400 ng/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MEP1A ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2 ng/ml -
Range
1.638 ng/ml - 400 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 82.58 74% - 92% Plasma 115.2 109% - 114% Cell culture media 112.5 79% - 141% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human MEP1A ELISA Kit is designed for the quantitative determination of MEP1A in cell culture supernatants, plasma and serum samples.
Δ Note: Human MEP1A concentration is low in normal serum/plasma and may not be detectable in this assay.
Δ Note: Collect plasma using EDTA and heparin as an anticoagulant. Citrate is not recommended as anticoagulants for use in this assay.
This assay employs an antibody specific for human MEP1A coated on a 96-well plate. Standards and samples are pipetted into the wells and MEP1A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MEP1A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MEP1A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Human MEP1A coated Microplate (12 x 8 wells) 1 unit Assay Diluent A 1 x 30ml Biotinylated Anti-Human MEP1A Detection Antibody 2 vials Human MEP1A Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Sequence similarities
Belongs to the peptidase M12A family.
Contains 1 EGF-like domain.
Contains 1 MAM domain.
Contains 1 MATH domain. -
Post-translational
modificationsN-glycosylated; contains GlcNAc, galactose, mannose and a small amount of fucose. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Endopeptidase 2
- Endopeptidase-2
- Mep1a
see all -
Database links
- Entrez Gene: 4224 Human
- Omim: 600388 Human
- SwissProt: Q16819 Human
- Unigene: 179704 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.