Human MELK knockout HCT116 cell line (ab266896)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Colon -
Cell type
epithelial -
Disease
Carcinoma -
Gender
Male -
STR Analysis
Amelogenin X D5S818: 10, 11 D13S317: 10, 12 D7S820: 11, 12 D16S539: 11, 13 vWA: 17, 22 TH01: 8,9 TPOX: 8, 9 CSF1PO: 7, 10 -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% DMSO, 2% Cellulose, methyl ether -
Research areas
Images
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Western blot - Human MELK knockout HCT116 cell line (ab266896)Anti-MELK antibody [EPR3981] (ab108529) at 1/1000 dilution + HCT116 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab108529 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab108529 was shown to react with MELK in HCT116 wild-type cells in western blot with loss of signal observed in MELK knockout cell line ab266896 ( MELK knockout cell lysate ab257537). HCT116 wild-type and MELK knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108529 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human MELK knockout HCT116 cell line (ab266896)Allele-1: 1 bp deletion in exon9
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Sanger Sequencing - Human MELK knockout HCT116 cell line (ab266896)Allele-2: Insertion of the selection cassette in exon 9.