Human MD2 ELISA Kit (ab277443)
Key features and details
- Sensitivity: 1.6 ng/ml
- Range: 1.638 ng/ml - 400 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MD2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.6 ng/ml -
Range
1.638 ng/ml - 400 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 94.92 80% - 109% Plasma 90.5 74% - 119% Cell culture media 103.3 95% - 111% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human MD2 ELISA Kit (ab277443) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MD2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human MD2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human MD2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human MD2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human MD2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 450X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Human MD2 Antibody 2 vials Anti-Human MD2 coated Microplate 1 unit Human MD2 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both MD2 and TLR4, but not TLR4 alone, respond to LPS. -
Post-translational
modificationsN-glycosylated; high-mannose. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- ESOP 1
- ESOP-1
- ESOP1
see all -
Database links
- Entrez Gene: 23643 Human
- Omim: 605243 Human
- SwissProt: Q9Y6Y9 Human
- Unigene: 726603 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.