Human MCAM (CD146) knockout HeLa cell lysate (ab256985)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260884 - Human MCAM knockout HeLa cell lysate 1 x 100µg ab255929 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human MCAM (CD146) knockout HeLa cell lysate (ab256985)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: MCAM knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab75769 observed at 120 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab75769 Anti-CD146 antibody [EPR3208] was shown to specifically react with CD146 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab261790 (knockout cell lysate ab256985) lane below 120kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and CD146 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab75769 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human MCAM knockout HeLa cell lysate (ab256985)Homozygous: 19 bp deletion in exon 6