Human Mannose Receptor ELISA kit (ab277420)
Key features and details
- Sensitivity: 0.41 ng/ml
- Range: 0.41 ng/ml - 100 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Mannose Receptor ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.41 ng/ml -
Range
0.41 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 104.6 83% - 131% Plasma 110.3 93% - 142% Cell culture media 109.3 91% - 127% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Mannose Receptor ELISA Kit (ab277420) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Mannose Receptor in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Mannose Receptor coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Mannose Receptor present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Mannose Receptor antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Mannose Receptor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Biotinylated Anti-Human Mannose Receptor Antibody 2 vials Anti-Human Mannose Receptor coated Microplate 1 unit Human Mannose Receptor standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. -
Sequence similarities
Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- bA541I19.1
- C type lectin domain family 13 member D
- C-type lectin domain family 13 member D
see all -
Database links
- Entrez Gene: 4360 Human
- Omim: 153618 Human
- SwissProt: P22897 Human
- Unigene: 75182 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.