Human LDHB (Lactate Dehydrogenase B) knockout HEK293T cell pellet (ab279082)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human LDHB knockout HEK293T cell pellet 1 vial Human wild-type HEK293T cell pellet 1 vial -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human LDHB (Lactate Dehydrogenase B) knockout HEK293T cell pellet (ab279082)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: LDHB knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab53292 observed at 37 kDa. Red - loading control ab130007 observed at 124 kDa.
ab53292 Recombinant Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] was shown to specifically react with LDHB in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab255403 (knockout cell lysate ab263761) was used. Wild-type and LDHB knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab53292 and Anti-Vinculin antibody [VIN-54] were incubated overnight at 4°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human LDHB (Lactate Dehydrogenase B) knockout HEK293T cell pellet (ab279082)Homozygous: 1 bp insertion in exon 2
