Human LBR (Lamin B Receptor) knockout HEK-293T cell lysate (ab257503)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260999 - Human LBR knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human LBR knockout HEK293T cell lysate (ab257503)
Lane 1: Wild-type HEK-293T cell lysate (20 µg)
Lane 2: LBR knockout HEK-293T cell lysate (20 µg)
Lane 3: Wild-type MEF-1 whole cell lysate (20 µg)
Lane 4: LBR knockout MEF-1 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab232731 observed at 58 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab232731 was shown to react with Lamin B Receptor (LBR) in wild-type HEK-293 and MEF-1 cells in western blot. Loss of signal was observed when LBR knockout samples were used. Wild-type and LBR knockout (ab257503) HEK-293 cell lysates and wild-type and LBR knockout MEF-1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab232731 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
LBR knockout MEF-1 samples were kindly provided by the Brian Burke laboratory, A-Star Institute, Singapore.
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Western blot - Human LBR knockout HEK293T cell lysate (ab257503)Lane 1: Wild-type HEK-293T cell lysate (20 µg)
Lane 2: LBR knockout HEK-293T cell lysate (20 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab32535 observed at 58 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab32535 was shown to react with Lamin B Receptor/LBR in wild-type HEK-293 cells in western blot. Loss of signal was observed when LBR knockout lysate ab257503 was used. Wild-type and LBR knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk before incubation with ab32535 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human LBR knockout HEK293T cell lysate (ab257503)Homozygous: 1 bp insertion in exon 2