Human KLK14 ELISA Kit (ab267634)
Key features and details
- Sensitivity: 1.5 pg/ml
- Range: 2.05 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human KLK14 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.5 pg/ml -
Range
2.05 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 122 101% - 143% Plasma 99.53 85% - 110% Cell culture media 98.38 92% - 105% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human KLK14 ELISA Kit is designed for the quantitative determination of KLK14 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human KLK14 coated on a 96-well plate. Standards and samples are pipetted into the wells and KLK14 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human KLK14 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of KLK14 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Human KLK14 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human KLK14 Detection Antibody 2 vials Human KLK14 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis. -
Tissue specificity
Highly expressed in CNS, bone marrow and fetal liver. Also expressed in breast, thyroid, kidney, colon, pancreas, spleen, prostate, uterus, small intestine, placenta and skeletal muscle. Among 40 tissues tested, the highest expression is detected in skin followed by breast and prostate (at protein level). Expressed in stratum corneum by sweat ducts and sweat glands and detected in sweat (at protein level). -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsProteolytic cleavage of the activation peptide produces the active enzyme. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- hK14
- Kallikrein 14
- Kallikrein like protein 6
see all -
Database links
- Entrez Gene: 43847 Human
- Omim: 606135 Human
- SwissProt: Q9P0G3 Human
- Unigene: 283925 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.