Human KDM6A (UTX) knockout HeLa cell pellet (ab278828)
Overview
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Product name
Human KDM6A (UTX) knockout HeLa cell pellet
See all KDM6A / UTX kits -
Product overview
Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.
Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation. -
Parental Cell Line
HeLa -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon7 and 2 bp deletion in exon7. -
Passage number
Knockout validation
Sanger Sequencing, Western Blot (WB)Notes
Pellet size: 5 million cells/vial.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Tested applications
Suitable for: WBmore detailsProperties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human KDM6A knockout HeLa cell pellet 1 vial Human wild-type HeLa cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Target
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Function
Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Demethylation of 'Lys-27' of histone H3 is concomitent with methylation of 'Lys-4' of histone H3, and regulates the recruitment of the PRC1 complex and monoubiquitination of histone H2A. -
Sequence similarities
Belongs to the UTX family.
Contains 1 JmjC domain.
Contains 8 TPR repeats. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- bA386N14.2
- bA386N14.2 (ubiquitously transcribed X chromosome tetratricopeptide repeat protein (UTX)
- bA386N14.2 ubiquitously transcribed X chromosome tetratricopeptide repeat protein UTX
see all
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human KDM6A knockout HeLa cell pellet 1 vial Human wild-type HeLa cell pellet 1 vial -
Research areas
-
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Images
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Western blot - Human KDM6A (UTX) knockout HeLa cell pellet (ab278828)
Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: KDM6A knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab36938 observed at 154 -157 kDa. Red - loading control ab8245 observed at 37 kDa.
ab36938 Anti-KDM6A / UTX antibody was shown to specifically react with KDM6A / UTX in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265110 (knockout cell lysate ab257214) was used. Wild-type and KDM6A / UTX knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab36938 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human KDM6A (UTX) knockout HeLa cell pellet (ab278828)Allele-1: 2 bp deletion in exon7
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Sanger Sequencing - Human KDM6A (UTX) knockout HeLa cell pellet (ab278828)Allele-2: 1 bp insertion in exon7