Human IRAK4 ELISA Kit (ab213472)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 247.4 pg/ml
- Range: 0.5 ng/ml - 32 ng/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IRAK4 ELISA Kit
See all IRAK4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% THP1 extract 8 5% Inter-assay Sample n Mean SD CV% THP1 extract 3 4% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
247.4 pg/ml -
Range
0.5 ng/ml - 32 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 111 107% - 115% Tissue Extracts 101 100% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IRAK4 ELISA Kit (ab213472) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IRAK4 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IRAK4 with 247.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
IRAK4 is a serine/threonine protein kinase involved in the innate immune response. IRAK4 is recruited by MD88 through TLR signalling, where it phosphorylates IRAK1 and ultimately results in NF-kappa-B nuclear translocation and activation.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (6 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IRAK4 Capture Antibody 1 x 600µl 10X Human IRAK4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BC 1 x 6ml Human IRAK4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1. -
Involvement in disease
Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. - Information by UniProt
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Alternative names
- IL-1 receptor-associated kinase 4
- Interleukin 1 receptor associated kinase 4 mutant form 1
- Interleukin-1 receptor-associated kinase 4
see all -
Database links
- Entrez Gene: 51135 Human
- Omim: 606883 Human
- SwissProt: Q9NWZ3 Human
- Unigene: 138499 Human
Images
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Other - Human IRAK4 ELISA Kit (ab213472)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IRAK4 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IRAK4 in human THP1 and PBMC extract based on a 500 µg/mL extract load.The concentrations of IRAK4 were measured in duplicate and interpolated from the IRAK4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IRAK4 concentration was determined to be 40.5 ng/mL in THP1 extract and 45.2 ng/mL in PBMC extract.
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Sandwich ELISA - Human IRAK4 ELISA Kit (ab213472)To learn more about the advantages of recombinant antibodies see here.
