Human Interferon omega ELISA Kit (ab119529)
Key features and details
- Sensitivity: 1.5 pg/ml
- Range: 4.7 pg/ml - 300 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Interferon omega ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 6 4.9% Inter-assay Sample n Mean SD CV% Overall 6 6.8% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.5 pg/ml -
Range
4.7 pg/ml - 300 pg/ml -
Recovery
91 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Interferon omega Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Interferon omega concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Human Interferon omega specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated Human Interferon omega detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of Human Interferon omega captured on the plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Assay Buffer Concentrate 1 x 5ml 20X Wash Buffer Concentrate 1 x 50ml Adhesive Films 4 units Biotin-Conjugate anti-Interferon omega monoclonal antibody 1 x 200µl Interferon omega Standard lyophilized (600 pg/mL upon reconstitution) 2 x 50µl Microplate coated with monoclonal antibody to Interferon omega (12 x 8 wells) 1 unit Sample Diluent 1 x 12ml Stop Solution (1M Phosphoric acid) 1 x 15ml Streptavidin-HRP 1 x 150µl TMB Substrate Solution 1 x 15ml -
Research areas
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Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IFNW1
- IFNW1_HUMAN
- Interferon alpha-II-1
- Interferon omega-1
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Database links
- Entrez Gene: 3467 Human
- SwissProt: P05000 Human
- Unigene: 73010 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab119529.