Human Immunoglobulin E ELISA Kit (IgE) (ab178659)
Key features and details
- Sensitivity: 0.27 IU/ml
- Range: 0 IU/ml - 200 IU/ml
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Immunoglobulin E ELISA Kit (IgE)
See all IgE kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 16 Inter-assay Sample n Mean SD CV% Serum 16 -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.27 IU/ml -
Range
0 IU/ml - 200 IU/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Immunogobulin E (IgE) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgE in serum and plasma.
The essential reagents required for an immunoenzymometric assay include high affinity and specificity antibodies. In this procedure, the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal anti-IgE antibody. Upon mixing monoclonal biotinylated antibody, and a serum containing the native antigen, reaction results between the native antigen and the antibody,forming an Antibody-Antigen complex. Simultaneously, the complex is deposited to the well through the high affinity reaction of streptavidin and biotinylated antibody. After a suitable incubation period, the antibody-antigen bound fraction is separated from unbound antigen by decantation or aspiration. Another antibody (directed at a different epitope) labelled with an enzyme is added. Another interaction occurs to form an Enzyme labelled Antibody-Antigen-Biotinylated-Antibody complex on the surface of the wells. Excess enzyme is washed off via a wash step. A suitable substrate is added to produce colour measurable with the use of a microplate spectrophotometer. The enzyme activity on the well is directly proportional to the native free antigen concentration. By utilizing several different serum references of known antigen concentration, a dose response curve can be generated from which the antigen concentration of an unknown can be ascertained.
Get higher sensitivity in only 90 minutes with Human IgE ELISA Kit (ab195216) from our SimpleStep ELISA® range.
-
Notes
IgE is an antibody isotype, found only in mammals. Although IgE is typically the least abundant isotype - blood serum IgE levels in a normal ("non-atopic") individual are ~150 ng/mL, compared to 10 mg/mL for the IgGs (the isotypes responsible for most of the classical adaptive immune response) - it is capable of triggering the most powerful immune reactions. Most of our knowledge of IgE has come from an allergy known as type 1 hypersensitivity.
-
Platform
Microplate
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 50X Washing Solution 1 x 20ml Cover Foil 1 unit IgE Biotin Conjugate 1 x 13ml IgE HRP Conjugate 1 x 13ml IgE Standard 0 - 0 mU/mL 1 x 1ml IgE Standard 1 - 5 mU/mL 1 x 1ml IgE Standard 2 - 25 mU/mL 1 x 1ml IgE Standard 3 - 50 mU/mL 1 x 1ml IgE Standard 4 - 150 mU/mL 1 x 1ml IgE Standard 5 - 400 mU/mL 1 x 1ml Stop Solution 1 x 15ml Streptavidin Coated Microplate (12 x 8 wells) 1 unit Strip holder 1 unit TMB Substrate Solution 1 x 15ml -
Research areas
-
Relevance
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock. -
Cellular localization
Secreted -
Alternative names
- Ig epsilon chain C region
- IgE
- IGHE
- immunoglobulin heavy constant epsilon
-
Database links
- Entrez Gene: 3497 Human
- Omim: 147180 Human
- SwissProt: P01854 Human
Images
-
Typical Standard Curve
Representative Standard Curve using ab178659.
