Human IL1R2 ELISA Kit (ab269548)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 24.96 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL1R2 ELISA Kit
See all IL-1R-2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 3 1.8% Inter-assay Sample n Mean SD CV% Serum 8 2.3% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
24.96 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 95 93% - 99% Serum 110 106% - 116% Hep Plasma 110 103% - 118% EDTA Plasma 110 105% - 118% Cit plasma 107 104% - 113% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL1R2 ELISA Kit (ab269548) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL1R2 protein in edta plasma, serum, urine, cit plasma, hep plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL1R2 with 24.96 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
IL1R2, interleukin 1 receptor, type II, is a key protein involved in the negative regulation of inflammatory and immune signaling. IL1R2 inhibits interleukins 1a and 1b and IL-1RAcp by binding to them without propagating a signaling cascade. Given its pivotal role in cell signaling, IL1R2 is currently under investigation for its role in a host of inflammatory and autoimmune-related pathologies. The antibodies for this kit were raised against a recombinant protein fragment of the mature human protein. This protein does not share a high sequence identity with mouse or rat and does not react. However, this kit does cross-react with monkey serum.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL1R2 Capture Antibody 1 x 600µl 10X Human IL1R2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IL1R2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1ra). -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- IL1 Receptor II
- Antigen CDw121b
- CD121
see all -
Database links
- Entrez Gene: 7850 Human
- Omim: 147811 Human
- SwissProt: P27930 Human
- Unigene: 25333 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IL1R2 standard curve in Sample Diluent NSThe IL1R2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL1R2 in human serum and plasma samples.The concentrations of IL1R2 were measured in duplicates, interpolated from the IL1R2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:20, plasma (citrate) 1:20, plasma (EDTA) 1:20, and plasma (heparin) 1:20. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1R2 concentration was determined to be 40.50 ng/mL in serum, 24.84 ng/mL in plasma (citrate), 30.33 ng/mL in plasma (EDTA), and 31.03 ng/mL in plasma (heparin).
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Interpolated concentrations of native IL1R2 in human urine samples.The concentrations of IL1R2 were measured in duplicates, interpolated from the IL1R2 standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1R2 concentration was determined to be 2.87 ng/mL in urine.
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Interpolated concentrations of native IL1R2 in human cell culture supernatant samples.The concentrations of IL1R2 were measured in duplicates, interpolated from the IL1R2 standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC cell culture supernatant 100% and unstimulated PBMC cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1R2 concentration was determined to be 226 pg/mL in stimulated PBMC cell culture supernatant and was undetectable in unstimulated PBMC cell culture supernatant.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL1R2 concentration was determined to be 43 ng/mL with a range of 31 – 77 ng/mL.
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Sandwich ELISA - Human IL1R2 ELISA Kit (ab269548)To learn more about the advantages of recombinant antibodies see here.
