Human IL-31 ELISA Kit (ab235637)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.5 pg/ml
- Range: 117.2 pg/ml - 7500 pg/ml
- Sample type: EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human IL-31 ELISA Kit
See all IL-31 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 2.5% Inter-assay Sample n Mean SD CV% Serum 3 1.7% -
Sample type
Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.5 pg/ml -
Range
117.2 pg/ml - 7500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 101 98% - 102% Cell culture media 105 103% - 109% EDTA Plasma 94 92% - 98% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-31 ELISA Kit (ab235637) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-31 protein in edta plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-31 with 1.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-31 Capture Antibody 1 x 600µl 10X Human IL-31 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human IL-31 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. -
Tissue specificity
Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- IL 31
- IL-31
- IL31
see all -
Database links
- Entrez Gene: 386653 Human
- Omim: 609509 Human
- SwissProt: Q6EBC2 Human
- Unigene: 569071 Human
Images
-
Other - Human IL-31 ELISA Kit (ab235637)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human IL-31 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
-
Example of human IL-31 standard curve in Sample Diluent 50BSBackground-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of spike IL-31 in human serum, plasma (EDTA), and cell culture media (RPMI containing 10% FBS) samplesThe concentrations of IL-31 were measured in duplicates, interpolated from the IL-31 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (EDTA) 25%, and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
-
Sandwich ELISA - Human IL-31 ELISA Kit (ab235637)To learn more about the advantages of recombinant antibodies see here.
