Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human IL-31 ELISA Kit (ab235637)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.5 pg/ml
  • Range: 117.2 pg/ml - 7500 pg/ml
  • Sample type: EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IL-31 ELISA Kit
    See all IL-31 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 5 2.5%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 1.7%

  • Sample type

    Serum, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.5 pg/ml

  • Range

    117.2 pg/ml - 7500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 101 98% - 102%
    Cell culture media 105 103% - 109%
    EDTA Plasma 94 92% - 98%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human IL-31 ELISA Kit (ab235637) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-31 protein in edta plasma and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-31 with 1.5 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human IL-31 Capture Antibody 1 x 600µl
    10X Human IL-31 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human IL-31 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent 50BS 1 x 20ml
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity.

  • Tissue specificity

    Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • IL 31
    • IL-31
    • IL31
    • IL31_HUMAN
    • Interleukin 31
    • Interleukin-31
    see all

  • Database links

    Images

    • Other - Human IL-31 ELISA Kit (ab235637)
      Other - Human IL-31 ELISA Kit (ab235637)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of human IL-31 standard curve in Sample Diluent NS
      Example of human IL-31 standard curve in Sample Diluent NS

      Background-subtracted data values (mean +/- SD) are graphed.

    • Example of human IL-31 standard curve in Sample Diluent 50BS
      Example of human IL-31 standard curve in Sample Diluent 50BS

      Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of spike IL-31 in human serum, plasma (EDTA), and cell culture media (RPMI containing 10% FBS) samples
      Interpolated concentrations of spike IL-31 in human serum, plasma (EDTA), and cell culture media (RPMI containing 10% FBS) samples

      The concentrations of IL-31 were measured in duplicates, interpolated from the IL-31 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (EDTA) 25%, and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    • Sandwich ELISA - Human IL-31 ELISA Kit (ab235637)
      Sandwich ELISA - Human IL-31 ELISA Kit (ab235637)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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