Human IL-25 ELISA Kit (ab272200)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 41.8 pg/ml
- Range: 94 pg/ml - 6000 pg/ml
- Sample type: Cell culture media, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-25 ELISA Kit
See all IL-25 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 8.4% Inter-assay Sample n Mean SD CV% Serum 4 14.8% -
Sample type
Serum, Cell culture media, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
41.8 pg/ml -
Range
94 pg/ml - 6000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 95 92% - 98% Cell culture media 113 108% - 115% Hep Plasma 87 86% - 87% EDTA Plasma 96 95% - 97% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-25 ELISA kit (ab272200) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-25 protein in serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-25 with 41.8 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin-25, also known as IL-17E, is a cytokine that belongs to the IL-17 cytokine family and is encoded by the IL25 gene. IL-25 has a heterodimeric receptor composed of IL-17RA and IL-17RB, where both subunits are necessary for IL-25 functions and are expressed in the main Th2 cells. The main function of IL-25 is to regulate the adaptive immune responses by induction of several cytokines, such as; IL-4, IL-5, and IL-13. IL-25 supports the Th2 immune response and has been found to play a role in protecting against bowel infections by various intestinal parasites.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-25 Capture Antibody 1 x 600µl 10X Human IL-25 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human IL-25 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. -
Tissue specificity
Expressed at low levels in several tissues, including brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea. -
Sequence similarities
Belongs to the IL-17 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IL 17E
- IL 25
- IL-17E
see all -
Database links
- Entrez Gene: 64806 Human
- Omim: 605658 Human
- SwissProt: Q9H293 Human
- Unigene: 302036 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IL-25 standard curve in Sample Diluent 50BP.The IL-25 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked human IL-25 in human serum, plasma and cell culture media samples.The concentrations of IL-25 were measured in duplicates, interpolated from the IL-25 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human IL-25 ELISA Kit (ab272200)To learn more about the advantages of recombinant antibodies see here.
