Human IL-22 ELISA Kit (ab216170)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.61 pg/ml
- Range: 13.12 pg/ml - 840 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-22 ELISA Kit
See all IL-22 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 4.6% Inter-assay Sample n Mean SD CV% Overall 3 2.6% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.61 pg/ml -
Range
13.12 pg/ml - 840 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 101 98% - 104% Urine 107 101% - 111% Serum 102 100% - 104% Hep Plasma 102 99% - 104% EDTA Plasma 102 100% - 105% Cit plasma 93 91% - 97% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human IL-22 ELISA Kit (ab216170) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-22 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-22 with 2.61 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin 22 (IL-22) is a cytokine thought to be involved in the response to inflammation through the Jak/STAT and MAPK signaling pathways. IL-22 is produced by NK cells and Th1-type T cells and can stimulate the induction of pro-inflammatory cytokines in human keratinocytes.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-22 Capture Antibody 1 x 600µl 10X Human IL-22 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human IL-22 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that contributes to the inflammatory response in vivo. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cytokine Zcyto18
- IL 10 related T cell derived inducible factor
- IL 21
see all -
Database links
- Entrez Gene: 50616 Human
- Omim: 605330 Human
- SwissProt: Q9GZX6 Human
- Unigene: 287369 Human
Images
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Other - Human IL-22 ELISA Kit (ab216170)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IL-22 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human IL-22 standard curve in Sample Diluent 50BS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked IL-22 in PBMC cell culture supernatant sample.The concentrations of IL-22 were measured in duplicates, interpolated from the IL-22 standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean spiked IL-22 concentration was determined to be 1015 pg/mL in PBMC supernatant.
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Interpolated concentrations of spike IL-22 in human serum, plasma and urine samples.The concentrations of IL-22 were measured in duplicates, interpolated from the IL-22 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, plasma (EDTA) 50% and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean spiked IL-22 concentration was determined to be 1174 pg/mL in serum, 1099 pg/mL in plasma (citrate), 1197 pg/mL in plasma (heparin), 1189 pg/mL in plasma (EDTA) and 1,322 pg/mL in urine.
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Human peripheral blood mononuclear cells were cultured unstimulated or stimulated with 10 µg/mL PHA.Conditioned media was harvested after 48 hours. IL-22 was measured in 50% unstimulated and PHA stimulated PBMC supernatant. The concentrations of IL-22 were measured in duplicate, interpolated from the IL-22 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-22 concentration was determined to be 316 pg/mL in PHA stimulated PBMC supernatant and 93 pg/mL in unstimulated supernatant.
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Sandwich ELISA - Human IL-22 ELISA Kit (ab216170)To learn more about the advantages of recombinant antibodies see here.
