Human IL-19 ELISA Kit (ab231922)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.648 pg/ml
- Range: 7.81 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-19 ELISA Kit
See all IL-19 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Milk 8 2.9% Inter-assay Sample n Mean SD CV% Milk 3 3% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.648 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 109 103% - 113% Saliva 106 105% - 108% Milk 100 98% - 103% Urine 83 81% - 86% Serum 89 86% - 90% Hep Plasma 89 88% - 92% EDTA Plasma 87 87% - 88% Cit plasma 86 86% - 88% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-19 ELISA Kit (ab231922) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-19 protein in cit plasma, edta plasma, hep plasma, milk, saliva, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-19 with 0.648 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-19 Capture Antibody 1 x 600µl 10X Human IL-19 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human IL-19 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May play some important roles in inflammatory responses. Up-regulates IL-6 and TNF-alpha and induces apoptosis. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IL 10C
- IL 19
- IL-19
see all -
Database links
- Entrez Gene: 29949 Human
- Omim: 605687 Human
- SwissProt: Q9UHD0 Human
- Unigene: 661017 Human
Images
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Other - Human IL-19 ELISA Kit (ab231922)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human IL-19 Competitor standard curve comparisonStandard curve comparison between Human IL-19 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human IL-19 standard curve in Sample Diluent NSThe IL-19 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked IL-19 in human serum and plasma samplesThe concentrations of IL-19 were measured in duplicate, interpolated from the IL-19 standard curves.
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Interpolated concentrations of native IL-19 in human milk (0.5%), saliva (10%), and PBMC supernatant (50%) samplesThe concentrations of IL-19 were measured in duplicate and interpolated from the IL-19 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-19 concentration was determined to be 37.14 ng/mL in milk, 91.95 pg/mL in urine, 779.30 pg/mL in saliva, and 1008.97 pg/mL in PBMC supernatant.
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. Interpolated concentrations of native IL-19 in human urine (25%), samplesThe concentrations of IL-19 was measured in duplicate and interpolated from the IL-19 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-19 concentration was determined to be 91.95 pg/mL in urine.
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Sandwich ELISA - Human IL-19 ELISA Kit (ab231922)To learn more about the advantages of recombinant antibodies see here.