Human IL-18BP ELISA Kit (ab224877)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.9 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-18BP ELISA Kit
See all IL-18BP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 3.5% Inter-assay Sample n Mean SD CV% Serum 3 3.3% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.9 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 94 87% - 102% Serum 115 103% - 122% Cell culture media 103 99% - 108% Hep Plasma 90 74% - 102% EDTA Plasma 86 78% - 90% Cit plasma 86 80% - 90% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human IL-18BP ELISA Kit (ab224877) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-18BP protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-18BP with 1.9 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin-18 binding protein is a secreted glycoprotein that functions as an IL-18 antagonist by preventing the interaction of IL-18 with IL-18 Receptor alpha. Isoform A of IL-18 Binding Protein binds to IL-18 and inhibits its activity. IL-18 Binding Protein functions as an inhibitor of the early TH1 cytokine response.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml 10X Human IL-18BP Capture Antibody 1 x 600µl 10X Human IL-18BP Detector Antibody 1 x 600µl Human IL-18BP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. -
Tissue specificity
Strongly expressed in heart, lung, placenta and spleen. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- I18BP_HUMAN
- IL-18BP
- IL18 binding protein
see all -
Database links
- Entrez Gene: 10068 Human
- Omim: 604113 Human
- SwissProt: O95998 Human
- Unigene: 591967 Human
Images
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Other - Human IL-18BP ELISA Kit (ab224877)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of HumanIL-18 Binding Protein standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-18 Binding Protein in Human serum and plasma samples.The concentrations of IL-18 Binding Protein were measured in duplicates, interpolated from the IL-18 Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 100%, plasma (heparin) 100%, plasma (EDTA) 100%, and urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 Binding Protein concentration was determined to be 3678 pg/mL in neat serum, 2682 pg/mL in neat plasma (citrate), 3360 pg/ml in neat plasma (heparin), 2957 pg/mL in neat plasma (EDTA), and 11,400 pg/ml in neat urine.
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Interpolated concentrations of spiked IL-18 Binding Protein in Human cell culture supernatant and media samples.The concentrations of IL-18 Binding Protein were measured in duplicates, interpolated from the IL-18 Binding Protein standard curves and corrected for sample dilution. Undiluted samples are as follows: THP-1 supernatant 100% and RPMI 1640 Media (containing 10% fetal bovine serum) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Serum from ten individual healthy Human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-18 Binding Protein concentration was determined to be 3678 pg/mL with a range of 3081 – 6405 pg/mL.
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Sandwich ELISA - Human IL-18BP ELISA Kit (ab224877)To learn more about the advantages of recombinant antibodies see here.
