Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human IL-17C ELISA Kit (ab279410)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.2 pg/ml
  • Range: 9.38 pg/ml - 600 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IL-17C ELISA Kit
    See all IL-17C kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 5.5%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5.2%

  • Sample type

    Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.2 pg/ml

  • Range

    9.38 pg/ml - 600 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 111 103% - 127%
    Serum 102 101% - 104%
    Hep Plasma 103 96% - 107%
    EDTA Plasma 114 109% - 117%
    Cit plasma 109 104% - 116%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human IL-17C ELISA kit (ab279410) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-17C protein in human serum, plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-17C with 1.2 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    IL-17C, also known as CX2, is a secreted cytokine of IL-17 family. IL-17C binds to a heterodimer formed by IL-17RA and IL-17RE. IL-17C plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. It stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NF-kappa-B and MAPK pathways. IL-17C is up-regulated by bacterial stimuli, including by lipopolysaccharides in gut epithelial cell lines, and by various proinflammatory cytokines, including TNF, IL-1B and IL-17F. IL-17C acts synergistically with IL-22 in inducing the expression of antibacterial peptides, and with TNF and IL-1B in inducing DEFB2 from keratinocytes. Depending on the type of insult, IL-17C may have both protective and pathogenic properties, either by maintaining epithelial homeostasis after an inflammatory challenge or by promoting inflammatory phenotype. It plays an important role in the pathogenesis of inflammatory arthritis.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Recombinant Antibody benefits
    Recombinant Antibody benefits

    To learn more about the advantages of recombinant antibodies see here.

  • Example of human IL-17C standard curve in Sample Diluent NS.
    Example of human IL-17C standard curve in Sample Diluent NS.

    The IL-17C standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native IL-17C in human HCT 116 cell culture supernatant sample treated with 15 ng/mL Hu IL-1ß for 24 hours.
    Interpolated concentrations of native IL-17C in human HCT 116 cell culture supernatant sample treated with 15 ng/mL Hu IL-1ß for 24 hours.

    The concentrations of IL-17C were measured in duplicates, interpolated from the IL-17C standard curves and corrected for sample dilution. Undiluted samples are as follows:  treated HCT 116 supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17C concentration was determined to be 93.5 pg/mL in neat treated supernatant. There was no detectable signal in mock treated HCT 116 cell supernatant.

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