Human IL-15RA ELISA Kit (ab264618)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.79 pg/ml
- Range: 23.44 pg/ml - 1500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-15RA ELISA Kit
See all IL-15RA kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.7% Inter-assay Sample n Mean SD CV% Serum 3 3.2% -
Sample type
Cell culture supernatant, Serum, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
5.79 pg/ml -
Range
23.44 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 90 81% - 96% Serum 115 107% - 121% Cit plasma 110 96% - 121% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-15RA ELISA Kit (ab264618) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-15RA protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-15RA with 5.79 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin-15 receptor subunit alpha is one of the three subunits that is part of its hematopoietin receptor family. IL-15RA contributes to IL-15 mediated effects such as cell survival, cell proliferation, and activation of T and B lymphocytes. This kit recognizes extracellular fragment of IL-15RA, which is solubilized and binds to IL-15 with high affinity. IL-15 does not interfere in ab264618.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-15RA Capture Antibody 1 x 600µl 10X Human IL-15RA Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 4BI 1 x 6ml Human IL-15RA Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for interleukin-15. Expression of different isoforms may alter or interfere with signal transduction. Isoform 5, isoform 6, isoform 7 and isoform 8 do not bind IL15. Signal transduction involves STAT3, STAT5, STAT6, JAK2 (By similarity) and SYK. -
Tissue specificity
Isoform 1, isoform 3, isoform 4, isoform 5, isoform 6, isoform 7, isoform 8 and isoform 9 are widely expressed. Expressed in fetal brain with higher expression in the hippocampus and cerebellum than in cortex and thalamus. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures. -
Sequence similarities
Contains 1 Sushi (CCP/SCR) domain. -
Post-translational
modificationsA soluble form (sIL-15RA) arises from proteolytic shedding of the membrane-anchored receptor. The cleavage involves ADAM17/TACE (By similarity). It also binds IL-15 and thus interferes with IL-15 binding to the membrane receptor.
Phosphorylated by activated SYK.
N-glycosylated and O-glycosylated. -
Cellular localization
Secreted > extracellular space; Membrane. Nucleus membrane. Mainly found associated with the nuclear membrane and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle membrane. Membrane. Isoform 5, isoform 6, isoform 7 and isoform 8 are associated with endoplasmic reticulum, Golgi and cytoplasmic vesicles, but not with the nuclear membrane. - Information by UniProt
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Alternative names
- AA690181
- CD215
- I15RA_HUMAN
see all -
Database links
- Entrez Gene: 3601 Human
- Omim: 601070 Human
- SwissProt: Q13261 Human
- Unigene: 524117 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IL-15RA standard curve in Sample Diluent NS + 1.5X Enhancer.The IL-15RA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-15RA in human serum and plasma.The concentrations of IL-15RA were measured in duplicates, interpolated from the IL-15RA standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-15RA concentration was determined to be 170.6 pg/mL in serum and 157.8 pg/mL in plasma (citrate).
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Interpolated concentrations of native IL-15RA in human cell culture supernatant samples.The concentrations of IL-15RA were measured in duplicates, interpolated from the IL-15RA standard curves and corrected for sample dilution. Undiluted samples are as follows: unstimulated PBMCs 50% and stimulated PBMCs 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-15RA concentration was determined to be undetectable in unstimulated PBMCs and 90.8 pg/mL in stimulated PBMCs.
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Sandwich ELISA - Human IL-15RA ELISA Kit (ab264618)To learn more about the advantages of recombinant antibodies see here.
