Human IgG1 ELISA Kit (ab100548)
Key features and details
- Sensitivity: 0.05 ng/ml
- Range: 0.05 ng/ml - 40 ng/ml
- Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IgG1 ELISA Kit
See all IgG1 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
0.05 ng/ml - 40 ng/mlRecovery
84 %
Sample specific recovery Sample type Average % Range Serum 87 75% - 97% Plasma 81.5 71% - 94% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s IgG1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IgG1 in serum and plasma.
This assay employs an antibody specific for Human IgG1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IgG1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IgG1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgG1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Notes
Optimization may be required with urine samples
Platform
MicroplateProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 2,000X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 2 x 15ml Biotinylated anti-Human IgG1 (lyophilized) 2 vials IgG1 Microplate (12 x 8 wells) 1 unit Recombinant Human IgG1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). -
Alternative names
- Ig gamma 1 chain C region
- IGHG1
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 2,000X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 2 x 15ml Biotinylated anti-Human IgG1 (lyophilized) 2 vials IgG1 Microplate (12 x 8 wells) 1 unit Recombinant Human IgG1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Relevance
There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). -
Alternative names
- Ig gamma 1 chain C region
- IGHG1
Images
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Sandwich ELISA - IgG1 Human ELISA Kit (ab100548)
Human IgG1 measured in biological fluids showing quantity (pg) per mL of tested sample. Human serum and plasma were diluted 1e6 - 64e6 fold. Human saliva and urine were diluted 1e3-64e3 fold.
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Typical standard curveRepresentative standard curve using ab100548