Human HRG ELISA Kit (ab277404)
Key features and details
- Sensitivity: 1.23 ng/ml
- Range: 1.229 ng/ml - 300 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human HRG ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% General Inter-assay Sample n Mean SD CV% General -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.23 ng/ml -
Range
1.229 ng/ml - 300 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 71.06 69% - 74% Serum 80.48 71% - 89% Plasma 77.28 69% - 99% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human HRG ELISA Kit (ab277404) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human HRG in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human HRG coated on a 96-well plate. Standards and samples are pipetted into the wells and Human HRG present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human HRG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human HRG bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Anti-Human HRG Antibody 2 vials Anti-Human HRG coated Microplate 1 unit Human HRG standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
HRG (histidine-rich glycoprotein) contains two cystatin-like domains and is located in plasma and platelets. The physiological function is not yet known. It binds heme, dyes and divalent metal ions. It can inhibit rosette formation and interacts with heparin, thrombospondin, and the lysine-binding site of plasminogen. A potential prothrombotic effect of HRG is indicated by the inhibition of fibrinolysis and the reduction of inhibition of coagulation. Mutations in HRG lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. -
Cellular localization
Secreted -
Alternative names
- DKFZp779H1622
- Histidine proline rich glycoprotein
- Histidine rich glycoprotein
see all -
Database links
- Entrez Gene: 3273 Human
- Entrez Gene: 3273 Human
- Omim: 142640 Human
- SwissProt: P04196 Human
- Unigene: 1498 Human
Images
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Human HRG ELISA kit (ab277404) Standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.