Human GOLPH2 ELISA Kit (ab277428)
Key features and details
- Sensitivity: 0.5 ng/ml
- Range: 0.573 ng/ml - 700 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human GOLPH2 ELISA Kit
See all GOLPH2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.5 ng/ml -
Range
0.573 ng/ml - 700 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 112.3 80% - 146% Plasma 103.1 74% - 110% Cell culture media 134.2 124% - 144% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human GOLPH2 ELISA Kit (ab277428) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human GOLPH2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human GOLPH2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human GOLPH2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human GOLPH2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human GOLPH2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Human GOLPH2 Antibody 2 vials Anti-Human GOLPH2 coated Microplate 1 unit Human GOLPH2 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Unknown. Cellular response protein to viral infection. -
Tissue specificity
Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocyte-derived syncitial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes. -
Sequence similarities
Belongs to the GOLM1/CASC4 family. -
Post-translational
modificationsGlycosylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Golgi apparatus > cis-Golgi network membrane. Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption. - Information by UniProt
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Alternative names
- bA379P1.3
- C9orf155
- Chromosome 9 open reading frame 155
see all -
Database links
- Entrez Gene: 51280 Human
- Omim: 606804 Human
- SwissProt: Q8NBJ4 Human
- Unigene: 494337 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.