Human Glypican 3 ELISA Kit (ab267640)
Key features and details
- Sensitivity: 0.8 ng/ml
- Range: 0.8 ng/ml - 600 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Glypican 3 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
0.8 ng/ml -
Range
0.8 ng/ml - 600 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 95 71% - 128% Plasma 75 69% - 85% Cell culture media 80 70% - 95% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Glypican 3 ELISA Kit is designed for the quantitative determination of Glypican 3 in cell culture supernatants, plasma and serum samples.
ΔNote: Human Glypican 3 concentration is low in normal serum/plasma and may not be detectable in this assay.
This assay employs an antibody specific for human Glypican 3 coated on a 96-well plate. Standards and samples are pipetted into the wells and Glypican 3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Glypican 3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Glypican 3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 1200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human Glypican 3 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human Glypican 3 Detection Antibody 2 vials Human Glypican 3 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. -
Tissue specificity
Highly expressed in lung, liver and kidney. -
Involvement in disease
Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1) [MIM:312870]; also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. -
Sequence similarities
Belongs to the glypican family. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
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Alternative names
- DGSX
- Glypican proteoglycan 3
- Glypican-3 [Precursor]
see all -
Database links
- Entrez Gene: 2719 Human
- Omim: 300037 Human
- SwissProt: P51654 Human
- Unigene: 644108 Human
Images
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Standard curve
Human Glypican 3 ELISA kit (ab267640) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.