Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Glutathione Peroxidase 1 ELISA Kit (ab193767)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 0.11 ng/ml
  • Range: 0.4 ng/ml - 25 ng/ml
  • Sample type: Cell culture extracts, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Glutathione Peroxidase 1 ELISA Kit
    See all Glutathione Peroxidase 1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4 4.17%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 9.8%

  • Sample type

    Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.11 ng/ml

  • Range

    0.4 ng/ml - 25 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 96 92% - 101%
    Hep Plasma 99 99% - 100%
    EDTA Plasma 93 91% - 98%
    Cit plasma 93 91% - 97%
    Extraction Buffer 96 91% - 100%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Glutathione Peroxidase 1 ELISA Kit (ab193767) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Glutathione Peroxidase 1 protein in cell culture extracts, cit plasma, edta plasma, hep plasma, serum, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Glutathione Peroxidase 1 with 0.11 ng/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    GPX1 is a selenium-containing enzyme part of the glutathione system responsible for protecting against oxidative damage by catalyzing the reduction of hydroperoxides with glutathione. The homotetramer, GPX1, utilizes its selenocysteine-containing active site to primarily scavenge hydrogen peroxide. The dimerization of glutathione ultimately provides the electrons to reduce the active site, restoring GPX1’s activity. As a critical component of the regulation of oxidative species within cells, GPX1 is directly involved in metabolism, various cancers, and oxidative stress. This assay is specific to isoform 1 of glutathione peroxidase.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

Images

  • Other - Human Glutathione Peroxidase 1 ELISA Kit (ab193767)
    Other - Human Glutathione Peroxidase 1 ELISA Kit (ab193767)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of Glutathione Peroxidase 1 standard curve.
    Example of Glutathione Peroxidase 1 standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Titration of Human Heart Homogenate (HHH), Human Liver Homogenate (HLH), HepG2, and HeLa extracts within the working range of the assay.
    Titration of Human Heart Homogenate (HHH), Human Liver Homogenate (HLH), HepG2, and HeLa extracts within the working range of the assay.

    Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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