Human Galectin 1 Matched Antibody Pair Kit (ab218170)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 125 pg/ml - 8000 pg/ml
Overview
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Product name
Human Galectin 1 Matched Antibody Pair Kit
See all Galectin 1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
3.97 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Galectin 1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Galectin 1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Galectin 1 Capture Antibody 2 x 50µg 1 x 50µg Human Galectin 1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Galectin 1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. -
Tissue specificity
Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. -
Sequence similarities
Contains 1 galectin domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- 14 kDa laminin-binding protein
- 14 kDa lectin
- Beta galactoside binding lectin
see all -
Database links
- Entrez Gene: 3956 Human
- Omim: 150570 Human
- SwissProt: P09382 Human
- Unigene: 445351 Human
Images
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Human Galectin 1 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Galectin 1 Matched Antibody Pair Kit (ab218170)To learn more about the advantages of recombinant antibodies see here.
